The prostaglandins of articular cartilage I. Correlates of prostaglandin activity in a chondrocyte culture system
Identifieur interne : 003806 ( Main/Exploration ); précédent : 003805; suivant : 003807The prostaglandins of articular cartilage I. Correlates of prostaglandin activity in a chondrocyte culture system
Auteurs : Michelle Copeland [États-Unis] ; Louis Lippiello [États-Unis] ; Grete Steensland [États-Unis] ; Walter C. Guralnick [États-Unis] ; Henry J. Mankin [États-Unis]Source :
- Prostaglandins [ 0090-6980 ] ; 1980.
English descriptors
- Teeft :
- Aliquot, Alkaline conversion, Alkaline extraction, Apparent leveling, Arachidonic, Arachidonic acid, Arachidonic acid supplementation, Articular, Articular cartilage, Assay, Calf chondrocytes, Cartilage, Cartilage metabolism, Cell density, Cell isolation, Cell isolation procedure, Chicken epiphyseal cartilage, Chondrocytes, Clinical assays, December, Endogenous cartilagenous, Ethanol, Ethanolic solution, Ethyl acetate, Exogenous, Fetal calf serum, Final concentration, Fresh medium, Grand island, Incubation, Indomethacin, Inflammatory disorders, Liquid scintillation, Lysosomal enzymes, Matrix material, Metabolism, Pge2, Prostaglandin, Prostaglandin synthetase inhibitors, Raven press, Rheumatoid arthritis, Rheumatoid synovium, Specific activity, Synthetase, Thin layer chromatography, Various cell densities, Various intervals.
Abstract
Abstract: Suspensions of aggregated chondrocytes display active prostaglandin (PG) production. Radioimmunoassay of culture media and thin layer chromatographic analysis suggests that PGE2 is the primary PG synthesized. In order of decreasing concentration, the following PG were tentatively identified; PGE > PGI > PGA + PGB ≧ PGF1+2 > T×B. An inverse logarithmic relationship was identified between PG synthesis and cells cultured at densities of 1.5 to 7.5 × 106 cells/ml. Little or no change in the PG distribution profile was seen at these high cell densities. Maximum PG synthesis was attained after 36 hours of incubation with persistence of high synthetic levels up to 48 hours. PGE2 production measured at various post-isolation intervals indicated an initial high rate of synthesis during the first 4 hours which decreased with time up to 24 hours. Cartilage explant organ cultures demonstrated a similar level of PG synthesis suggesting minimal effect of matrix on cellular PG production. Indomethacin (5 μg/ml) inhibited PG synthesis by 70% within 4 hours and 85% after 24 hours of exposure. Arachidonic acid supplementation (10 μM) stimulated PG synthesis by 300%.
Url:
DOI: 10.1016/0090-6980(80)90061-1
Affiliations:
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Correlates of prostaglandin activity in a chondrocyte culture system</title><author><name sortKey="Copeland, Michelle" sort="Copeland, Michelle" uniqKey="Copeland M" first="Michelle" last="Copeland">Michelle Copeland</name><affiliation wicri:level="3"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Orthopaedic Research Laboratory and Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital and Harvard Medical School, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Lippiello, Louis" sort="Lippiello, Louis" uniqKey="Lippiello L" first="Louis" last="Lippiello">Louis Lippiello</name><affiliation wicri:level="3"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Orthopaedic Research Laboratory and Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital and Harvard Medical School, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Steensland, Grete" sort="Steensland, Grete" uniqKey="Steensland G" first="Grete" last="Steensland">Grete Steensland</name><affiliation wicri:level="3"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Orthopaedic Research Laboratory and Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital and Harvard Medical School, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Guralnick, Walter C" sort="Guralnick, Walter C" uniqKey="Guralnick W" first="Walter C." last="Guralnick">Walter C. Guralnick</name><affiliation wicri:level="3"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Orthopaedic Research Laboratory and Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital and Harvard Medical School, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Mankin, Henry J" sort="Mankin, Henry J" uniqKey="Mankin H" first="Henry J." last="Mankin">Henry J. Mankin</name><affiliation wicri:level="3"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Orthopaedic Research Laboratory and Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital and Harvard Medical School, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Prostaglandins</title><title level="j" type="abbrev">PROOLD</title><idno type="ISSN">0090-6980</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1980">1980</date><biblScope unit="volume">20</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1075">1075</biblScope><biblScope unit="page" to="1087">1087</biblScope></imprint><idno type="ISSN">0090-6980</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0090-6980</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aliquot</term><term>Alkaline conversion</term><term>Alkaline extraction</term><term>Apparent leveling</term><term>Arachidonic</term><term>Arachidonic acid</term><term>Arachidonic acid supplementation</term><term>Articular</term><term>Articular cartilage</term><term>Assay</term><term>Calf chondrocytes</term><term>Cartilage</term><term>Cartilage metabolism</term><term>Cell density</term><term>Cell isolation</term><term>Cell isolation procedure</term><term>Chicken epiphyseal cartilage</term><term>Chondrocytes</term><term>Clinical assays</term><term>December</term><term>Endogenous cartilagenous</term><term>Ethanol</term><term>Ethanolic solution</term><term>Ethyl acetate</term><term>Exogenous</term><term>Fetal calf serum</term><term>Final concentration</term><term>Fresh medium</term><term>Grand island</term><term>Incubation</term><term>Indomethacin</term><term>Inflammatory disorders</term><term>Liquid scintillation</term><term>Lysosomal enzymes</term><term>Matrix material</term><term>Metabolism</term><term>Pge2</term><term>Prostaglandin</term><term>Prostaglandin synthetase inhibitors</term><term>Raven press</term><term>Rheumatoid arthritis</term><term>Rheumatoid synovium</term><term>Specific activity</term><term>Synthetase</term><term>Thin layer chromatography</term><term>Various cell densities</term><term>Various intervals</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Suspensions of aggregated chondrocytes display active prostaglandin (PG) production. Radioimmunoassay of culture media and thin layer chromatographic analysis suggests that PGE2 is the primary PG synthesized. In order of decreasing concentration, the following PG were tentatively identified; PGE > PGI > PGA + PGB ≧ PGF1+2 > T×B. An inverse logarithmic relationship was identified between PG synthesis and cells cultured at densities of 1.5 to 7.5 × 106 cells/ml. Little or no change in the PG distribution profile was seen at these high cell densities. Maximum PG synthesis was attained after 36 hours of incubation with persistence of high synthetic levels up to 48 hours. PGE2 production measured at various post-isolation intervals indicated an initial high rate of synthesis during the first 4 hours which decreased with time up to 24 hours. Cartilage explant organ cultures demonstrated a similar level of PG synthesis suggesting minimal effect of matrix on cellular PG production. Indomethacin (5 μg/ml) inhibited PG synthesis by 70% within 4 hours and 85% after 24 hours of exposure. Arachidonic acid supplementation (10 μM) stimulated PG synthesis by 300%.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region><settlement><li>Boston</li></settlement></list><tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Copeland, Michelle" sort="Copeland, Michelle" uniqKey="Copeland M" first="Michelle" last="Copeland">Michelle Copeland</name></region><name sortKey="Guralnick, Walter C" sort="Guralnick, Walter C" uniqKey="Guralnick W" first="Walter C." last="Guralnick">Walter C. Guralnick</name><name sortKey="Lippiello, Louis" sort="Lippiello, Louis" uniqKey="Lippiello L" first="Louis" last="Lippiello">Louis Lippiello</name><name sortKey="Mankin, Henry J" sort="Mankin, Henry J" uniqKey="Mankin H" first="Henry J." last="Mankin">Henry J. Mankin</name><name sortKey="Steensland, Grete" sort="Steensland, Grete" uniqKey="Steensland G" first="Grete" last="Steensland">Grete Steensland</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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